Collect your 48 hpf embryos and dechorionate them (either by using tweezers or enzyme digestion) and let them acclimatize for 1 hour. Ideally use embryos that have pigment, rather than a pigment-free strain, as it helps to isolate the fish during analysis later on. Make sure you do not have reflections of the lab lights in your dish as it makes analysis difficult – you want homogenous illumination of the dish. Set up a clamp stand holding your camera above the Petri dish, at whatever height allows you to see the edges of the Petri dish on the screen. Place your Petri dish containing medium onto the heated stage or benchtop, ensuring you have a white surface underneath – a few sheets of white paper does the job.
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